Format must be FASTA: >your_descriptionATGCATGC... the nucleic sequence (16S, 23S will hopefully come soon)
OR #Genus_species :
Paste the query here (or ? or help) then proceed to the next step
The default DB is the "reference non redundant sequence DB" containing one sequence per species. These sequences are selected on their quality and representativity.
You cannot add a limited number of sequences in the highest interest zone of the phylogenetic tree (consider using the optimized phylogeny entry).
Experimental DB : Bacteria-DNA-directed-RNA-polymerase (rpoB) DB and Corynebacteriales-Chaperonine-groEL DB are available but their integration in the results pages may not be optimal. Note that the ↑ in the tree give access to the 16S tree.
The default number if sequences is 100, you may increase it up to 400
The default ALIGNER is kalign
The default trimming tool is only removing columns with more than 45% gaps
Trying to mitigate low quality positions of the query is the default (mitigation not available if Gblocks is the trimmer)
Polytomy when nodes SH level under 0.2 is the default
Rooting by minimal variance is the default