Whith Chromium derivated browsers the alignment is slightly shifted, unfortunately this cannot be patched on our side
For the impatient
engine is a set of programs to identify antibiotic resistance mutations using nucleic acid sequences
MUBII-TB is the database of mutations leading to antibiotic resistance and their corresponding reconstructed sequences for
Press the button [
Run and wait
] to use the default settings using a test sequence and see what happens
Why do we need such a web site
is a bacterial infectious disease due to
It remains a major health threat, killing over a million people every year, worldwide.
An early antibiotic therapy is the basis of the treatment.
The emergence and spread of multidrug and extensively drug-resistant mutant strains raise significant challenges for the patients and others.
As these bacteria grow very slowly, the detection of drug resistance mutations is currently done by using molecular biology. Resistance mutations are identified by sequencing of the resistance-linked genes, followed by comparison with literature data.
The only online database is TBDReaMDB, but it requires mutation detection before use, and its interrogation is complex because we website is not interactive.
The same problem occurs for some other bacteria like
the agent of lepra
Some more explanations
Choosing your database
The only database concern
resistance to antibiotics, other databases will come
MUBII-TB is a structured database of the mutations occurring in
(select one) of major therapeutic value for tuberculosis management.
Try with your data
Copy the sequences (in fasta format) in the "Query" section.
You can try with any of the reference sequences (see the lateral menu in the query form))
Press the button [Run and wait] to use the default settings using a test sequence
This is a way to test MUBII (useful if MUBII does not work well with your submission)
You get the results of the computation in the next window
How it works
A highly structured, simple text-based database, contains a description of the mutations (DNA and proteins) toward antibiotic resistance for a given species of bacteria (for instance the MUBII-TB-DB concern
and seven loci: rpoB, pncA, katG; mabA(fabG1)-inhA, gyrA, gyrB, rrs.
Resistance mutation data are extracted after systematic review of MEDLINE referenced publications.
MUBII is the tool that analyses the query sequence obtained by PCR-sequencing
A BLAST search is done against a set of previously reconstructed mutated sequences;
Alignment of the query sequences (DNA and its protein translation) is done against the wild-type sequences.
The post-treatment includes the extraction of the aligned sequences together with their descriptors (position and nature of mutations).
The whole process is done on the web. The results are graphs (alignments) and text (description of the mutation, therapeutic significance).
A printed result may be generated.
Moreover, the system provides interpretation in biological and therapeutic terms and can evolve by the addition of newly described mutations.
Its interest is to give an easy and comprehensive access to an up-to-date database of mutations leading to the resistance of bacteria (for instance
) to antibiotics through a client-server model over the web.
The system is rapid and easy to use, even by people without bioinformatics formation.